液相色谱质谱联用仪
仪器介绍
nanoElute 2基于前几代的核心技术进行了优化，得以实现无与伦比的稳健性、终极色谱性能和易用性。nanoElute 2 可0覆盖从单细胞到高通量得常规蛋白质组学的广泛应用领域。这个新一代系统可提供最高的色谱分辨率，从而实现出色的肽鉴定和定量分析。Elute LC 系统建立在一种创新的泵设计之上，提供独立于流速、溶剂可压缩性或成分得几乎无脉冲式、精确的流速流量控制性能。
所有 Elute LC 系统配置有两对串联的、独立控制的线性驱动泵头。这一设计使得相邻实验室或全球范围内不同系统间的超高性能液相色谱方法转移变得容易。通过最小化梯度延迟体积对液体流路路径进行优化，从而实现高色谱分辨率、快速梯度和最短循环时间，提高样品通过量并降低成本。
timsTOF HT 质谱仪配备了第四代高容量TIMS-XR 分析器和先进的数字化技术（ADT）。它提供了更高的动态范围，在高通量蛋白质组学实验中可实现无与伦比的分析深度和定量分析。timsTOF HT 具有离子淌度功能，可预测CCS值，并支持所有 PASEF® 采集模式，包括 PASEF®、dia-PASEF® 和 prm-PASEF®，实现了 4D-Proteomics™ 的灵活性，在大规模上实现无可妥协的蛋白质组深度。 timsTOF 平台以其卓越的可靠性和最小的维护需求脱颖而出，使其理想地适用于无缝集成到日常实验室工作流程中，最大程度地提高正常运行时间并实现快速周转。在只有 5 分钟的梯度时间内，在多个实验室轻松鉴定超过 7000 个蛋白质群，可重复性极高，无需手动干预。
timsTOF 平台以其卓越的可靠性和最小的维护需求脱颖而出，使其理想地适用于无缝集成到日常实验室工作流程中，最大程度地提高正常运行时间并实现快速周转。在只有 5 分钟的梯度时间内，平均可以轻松在多个站点识别超过 7000 个蛋白质群。实现随时间的顶级可重复性，无需手动干预。
参数
| nanoElute | |
| Sample loop | 20 µL |
| Sample loading range | 1-10 µL（Partial loop）or 20 µL (full loop) |
| Sample tray | 2 sample plate (54 position/plate) or 96-well plate or 384-well plate |
| Sampler Temperature range | 15℃ below room temperature |
| Single-acting syringe pump | |
| Flow | 50-2000 nL/min |
| Pressure of loading pump | < 1000 bar |
| Column compartment temperature | 25-60℃ |
| Elute | |
| High-pressure mixing binary pump | max. 1300 bar |
| Flow | 1-5000 µL/min |
| Mixer volume | 35 µL |
| Sample loading mode | Full-loop/partial-loop/µL-pickup |
| Sample tray | 1 sample plate (108 position for 2mL-vial or 30 position for 10mL-vial) or 96-well plate or 384-well plate |
| Sampler temperature range | 4-40℃ |
| Column compartment temperature | 15-90℃ |
| timsTOF HT | |
| Scan rate | MS ≥ 50 Hz; Up to 300 Hz in dda-PASEF |
| MS range | Up to 20000 m/z |
| Resolution | 60000 (1222 m/z) |
| Mass accuracy | Internal ≤ 0.8 ppm (tuning mix m/z 1221.990637, 30 spectral)；External ≤ 2 ppm (tuning mix m/z 1221.990637, 10 spectral) |
| Sensitivity | 1pg/µL Reserpine (m/z 609), S/N 100:1 |
| Features | Trapped Ion Mobility Spectrometry (TIMS) is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF. |