高通量光谱位移亲和力筛选仪
仪器介绍
Dianthus是一个基于平板且无微流体的亲和筛选平台。在溶液中检测，且不依赖于质量，非常适合于条件严格的筛选实验。
参数
| Biophysical modalities | Spectral Shift and TRIC |
| Format | 384-well plate (barcoded and sealable) |
| Affinity range | pM to mM |
| Data points per run | Up to 384 |
| Run time for 384 data points | 33 minutes (Spectral Shift), 79 minutes (Spectral Shift + TRIC) |
| Time it takes to get a Kd 12-point dilution series | 60 seconds (Spectral Shift), 132 sec (Spectral Shift + TRIC) |
| Sample volume/well | 20 µL |
| Molecular weight range | 10*^1-10*^7 Da |
| Temperature control | 20-25 °C +/- 0.5 °C (actively controlled) |
| Compounds tested in 24 h (single dose, in duplicate, plate feeder) | 7400 |
| Kds measured in 24 h (12-point dilution series, plate feeder) | 1350 |