流式细胞分选仪
仪器介绍
BD FACSDiscover™S8细胞分选机采用BD CellView™图像技术和BD SpectralFX™技术，是具有图像辅助分选的开创性光谱流式细胞仪分选机。这项革命性的技术通过将光谱流式细胞术与实时空间和形态学分析相结合，扩展了细胞分析和分选的能力，使科学家能够着手解决以前无法回答的问题。它可以在分析和分选过程中实时观察细胞群体的图像，通过空间和形态学分析改进光谱流式细胞术，从而识别和分离此前无法获得的细胞群。同时，它还通过综合视觉观测、灵活的面板设计和自动化功能简化了实验操作流程。它还通过将下游分析与成像和传统流量数据相关联，从而创建高度复杂的疾病和系统的综合图谱。
参数
| Lasers | 349 nm (30 mW) 405 nm (50 mW) 488 nm (100 mW) 561 nm (50 mW) 637 nm (100 mW) |
| Scatter detectors | Blue laser: Forward side scatter (FSC) Side scatter (SSC) Axial light loss (ALL) Violet laser: Axial light loss (ALL) Side scatter (SSC) |
| Fluorescence detectors | UV 349-nm laser (22 UV detectors, covering 365–860 nm) Violet 405-nm laser (20 Violet detectors, covering 410–860 nm) Blue 488-nm laser (16 Blue detectors, covering 495–860 nm) Y/G 561-nm laser (12 Yellow-green detectors, covering 570–860 nm) Red 637-nm laser (8 Red detectors, covering 645–860nm) |
| Image-enabled detectors | Blue laser scatter detectors: Forward scatter (FSC) Side scatter (SSC) Axial light loss (ALL) Fluorescence detectors for imaging (PMTs): FL1: LP505: 534/46 FL2: LP570: 600/60 FL3: LP675: 788/225 |
| Imaging features | Center of mass X Center of mass Y Correlation Delta center of mass Diffusivity Eccentricity Max intensity Moment (long) Moment (short) Radial moment Size Total intensity |
| Nozzle size | 85/100/130 µm |
| Sort collection devices | Tube sorting: 1.5-mL microtubes, 2.0-mL tubes and 5.0-mL 12x75-mm tubes Plate sorting: 6-, 24-, 48-, 96- and 384-well plates, microscope slide (optional) |