细胞能量代谢分析系统

型号:

Seahorse XF HS Mini

地点:

127, 1F, E4

仪器介绍

Seahorse XF HS Mini能量代谢分析系统，利用非侵入、无标记式传感器，同时、实时测量活细胞的两条能量代谢通路，量化细胞代谢表型，从而分析细胞功能的变化。 仪器通过测定氧气消耗速率（ Oxygen Consumption Rate, OCR）来反映细胞线粒体的功能，通过测定细胞外酸化速率（ Extra Cellular Acidification Rate, ECAR）、质子流出速率（Proton Efflux Rate,  PER）来反映糖酵解功能。

参数

Analyzer applicationBasal metabolic rate; Limiting respiratory rate; Respiratory reserve capacity; Proton leakage level, etc
Sample typesSuspension cells; Adherent cells; Primary cell; Isolated mitochondria;
Microchamber volume2.8 µL
Sample temperature16 - 40 °C
Sample requirements5,000 - 500,000 cells/well
Assay wells6
Assay running volume150 - 275 µL/well
Volume per inlet25 µL
Inlet per well4 per well

手册

Seahorse XF HS Mini_Brochure_EN