电穿孔仪
仪器介绍
ECM830配置有安全圆顶,能够有效转染真核细胞、原核细胞和植物原生质体等。
参数
Voltage range | LV mode: 5-500V in increments of 1 V HV mode: 505-3000 V in increments of 5 V |
Voltage accuracy | LV Mode: 5% HV Mode: 5% |
Pulse length range | LV Mode: 10-999 μs in increments of 1 μs;1-999 ms in increments of 1 ms HV Mode: 10-600 μs in increments of 1 μs |
Multiple pulsing | 1 to 99 (per individual sample) or 1 to 120 (10 per sample, with HT plate handler) |
Pulse interval | 0.1 s to 10 s |
Capacitance in μF | LV Mode: 3775 HV Mode: 85 |
Voltage droop | 20% Load <1 kΩ, 10% Load >1 kΩ |
Sample resistance (Load) | LV Mode: PL ≤ 100 ms, Load ≥ 8 to 9 Ω; PL >100 ms, Load ≥ 100 Ω HV Mode: Load ≥ 40 Ω |
Charging time | LV < 7 s, HV < 4 s |
Monitoring | Pulse Voltage, Width, Droop% and Sample Resistance |
Display | 7.0-in color display |
Controls | Touch Screen |
Programmability | Storage over 1,000 Protocols |
Applicatoins | Mammalian cell transfection,In vitro, in vivo, ex vivo and in ovo tissue transfection, Nuclear transfer, Ex plant tissue transfection, Protoplast transfection, siRNA libraries, High throughput 25- and 96-well electroporation, CRISPR transfections |
Compatible BTX cuvettes | 1 mm, 2 mm, and 4 mm gap BTX cuvettes |
Safety dome | Holds two cuvettes, Safety swing shield secures cuvettes in holder, Operating Temperature 5 to 40°C |