高内涵成像分析筛选系统

仪器介绍

CellVoyager CV8000高含量筛选系统集成了高速共焦扫描仪技术、水浸透镜、多达四个高视野相机、带细胞培养环境的显微镜载物台和集成机器人移液器，该系统提供高通量、高分辨率成像和表型筛选，是广泛应用的理想选择。

参数

Main unit	• Microlens enhanced dual wide Nipkow disk confocal （pinhole size：25μm/50μm） • 405nm/445nm/488nm/561nm/640nm laser • 365nm LED Illumination • Standerd sCMOS X 4 - 4 mega pixel (2000x2000) - 16 bit resolution - 6.5 μm pixel size - supports fast recording mode with up to 100 fps - QE≥82% • Sample vessel for Microplate(6,24,96,384,1536 well) • Laser autofocus • Software autofocus • CV8000 software(Measurement and analysis) • Bright Field/Phase Contrast • Chambered Type Stage Attachment • 6 objective lens position holder
Objective lens	• 4xDry(NA=0.16)UPLXAPO4X • 10xDry(NA=0.40)UPLXAPO10X2 • 20xDry(NA=0.80)UPLXAPO20X • 40xDry(NA=0.95)UPLXAPO40X2 • 20x Water (NA=1.00 UPLSAPO20XW • 60x Water (NA=1.2) UPLSAPO60XW
Dispenser	• For 384-tip Type - Dispenser, - Tip Rack Loader, - Source Shuttle
Living cell system	• Stage heater • Internal Gas mixture（CO2 & N2） • Humidity maintenance system
Filter	• 5-position filter wheel for each sCOMS • EM 445/45 for 405nm • EM 525/50 for 488nm • EM 600/37 for 561nm • EM 676/29 for 640nm • EM 483/32 for CFP • EM 539/30 for YFP
Advanced imaging control module	• Cell Voyager ACE The Cell Voyager ACE module enable a fully automated workflow of low magnification pre-scan, image analysis and higher magnification rescan.This workflow can be used, for example, to locate 3D organoids in all wells of a multi-plate, then acquire a z-stack at high power objective lens in the center of the tissue. • Fast Time-lapse module (up to 45fps) This module enables ultra-high speed continuous fluorescence imaging of a specific field of view and can be applied to time lapse photography of rapid biological reactions such as calcium ion flow, calcium spark, ion channel rapid response, etc
Barcode reader & Sample holder	• For Slide Glass，4 slides (ISO 8037/1)
Software & Workstations	• Standerd WS with CV8000 control system • Advanced Professional image analysis workstation • Cell Path finder High content software -3D cell segmentation module -Aplication mode/ Template mode for different analysis needs -Basic / Advanced cell segmentation mode -Opening algorithms：Custom image analysis • Digital phase contrast analysis module • Machine learning module -Cell recognition mode -Object recognition mode -Up to 10 graphic libraries • Cell tracking module • Deep Learning license -Deep Area Finder -Deep Detection -Deep Image Gate -Deep Image Response

手册

Cell Voyager CV8000_Brochure_EN