大组织样本光片显微成像系统
仪器介绍
LiTone XL利用线性贝塞尔光片技术和折射率矫正光学模组，提供可达500nm的成像分辨率，搭配四侧照明技术，可实现大型透明化样品的快速三维成像，包括透明化的组织、器官及胚胎等，同时也可对多个小型活体样品（例如斑马鱼，果蝇胚胎）进行长时间的成像。
参数
| Lasers | 405nm (DAPI), 488nm (GFP) 561nm (mCherry) 647nm (Alexa647) |
| Available objectives | * 4x/0.28 Air lens, WD 28.8-30.0mm * 10x/0.6, adaptable RI 1.33-1.52, WD 8mm * 25x/1.0, adaptable RI 1.41-1.52, WD 8mm * 16x/0.8 Water lens, WD 3mm |
| Field of view | 3x3mm/1.2x1.2mm FOV for 4X/10X objectives or be multiplied by stitching |
| Illumination mode | Four-side/two-side/single-side illumination with Line Bessel Sheet (LBS) technology |
| Sample size | From 200μm to 20 mm diameter |
| Detection camera | sCMOS camera, 16bits, 2048 by 2048 pixels full frame, 6.5μm pixel size; Peak quantum efficiency > 82% @ 560nm |
| Detection speed | 80 frame per second at 2048 x 2048 frame size |
| Spatial resolution | For 10X air objective: 0.6μm lateral by 1.5 μm axial (with light-sheet mode 2) |
| Sample mounting | Transparent-well mounting for cleared tissue samples; FEP or agarose mounting for live or fixed embryos |
| Sample positioning | Full motorized high-precision XYZ sample stages |