活样本光片显微成像系统
仪器介绍
LiTone LBS2利用超薄的线性贝塞尔片层光于物镜焦面激发样品，可实现低光毒性、高速、高分辨率的活细胞三维结构成像，横向分辨率可达250nm，拍摄速度可达500幅每秒，可观测的样本包括活的贴壁细胞或组织、胚胎或小动物以及悬浮细胞或微生物。
参数
| Lasers | 405nm (DAPI), 488nm (GFP) 561nm (mCherry) 647nm (Alexa647) |
| Detection objectives | 25x/1.1 Water-immersion lens, WD 2 mm (with 1x & 2.5x optical zoom) |
| Field of view | Single view: 200μm X 200μm (automatic stitching for wide-field range imaging) |
| Sample size | 20 mm X 20 mm X 2 mm |
| Max data acquisition speed | 80 frames per second (2048 X 2048 full frame) |
| Resolution | Lateral resolution: 250nm Axial resolution: 350nm |
| Time resolution | 2 Hz per channel @ sample size of 200 μm X 30 μm X 30 μm |
| Adaptable samples | Fixed or live adherent cells or tissues Embryos or small animals Suspended cells or microorganisms |
| Loading method | horizontal sample loading method |
| Sample stage | High-precision three-axis motorized stage |
| Imaging depth | Typically 30-1500 μm, determined by the optical properties of the samples |
| Environmental control module | Integrated live cell culture chamber, temperature control range: 25-40 ºC, 5% CO2 inlet |