MALDI TOF/TOF质谱
仪器介绍
rapifleX系统结合了最尖端的技术，具有完美的速度、稳健性和可性度。rapifleX平台以无以伦比的速度和性能在蛋白表征、生物标志物发现、聚合物分析、高通量筛选、糖肽分析和组织成像等方面提供高质量分析。
参数
| Ion source | MALDI ion source |
| Laser type | BRUKER scanning smartbeam™ 3D laser, 355 nm wavelength |
| Laser frequence | 0.001 kHz-10 kHz |
| Number of laser shots | 1*1010 (10 billion) |
| Mass Resolution | Linear mode ≥ 1,200 for Cytochrome C (m/z 12,361) FWHM； Reflect mode ≥ 50,000 for Somatostatin 28 (m/z 3,147.5) FWHM; MS/MS Fragment Resolution，≥ 1,500 for [Glu1]-Fib fragment m/z 175, ≥ 5,000 for [Glu1]-Fib fragment m/z 684, ≥ 7,500 for [Glu1]-Fib fragment m/z 1,056, ≥ 10,000 for [Glu1]-Fib fragment m/z 1,441 |
| MS Sensitivity | Linear mode, 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots；Reflect mode , 250 amol [Glu1]-Fibrinopeptide B (m/z 1,570.7) at S/N ≥ 200:1 shown with 2,000 laser shots; MS/MS mode: 250 amol [Glu1]-Fibrinopeptide B fragment 1,056 Da at S/N ≥ 30:1 shown with 1,000 laser shots |
| Linear mode MS accuracy | Protein mixture ≤ 60 ppm with external calibrant, ≤ 50 ppm with internal calibrant |
| Reflect mode MS accuracy | Protein mixture ≤ 5 ppm with external calibration, ≤ 1.0 ppm with internal calibration |
| MS/MS mass accuracy | ≤ 0.025 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570) |
| Imaging pixel shape and size | Squared pixel for best tissue coverage with variable pixel size of 20 µm to 100 µm |
| Imaging speed | Up to 40 pixel/sec |