多功能蛋白稳定性分析仪
仪器介绍
Prometheus Panta可以让您能够在一次运行中测量样品的热稳定性、聚集性、颗粒尺寸和分散性——无需标记，也无需使用大量样本。从Prometheus Panta这里可获得可靠的高分辨率蛋白质稳定性数据。
参数
| nanoDSF | Measurement parameters: ratio: Tonset, Tm, Ea, reversibility of unfolding 330 nm, 350 nm: Tm, Excitation: 280 nm; Concentration range: 0.005 mg/mL – 250 mg/mL; Inflection point precision @ 75 °C: ± 0.1 °C; Initial ratio repeatability: 0.008. |
| DLS | Measurement parameters: Tscattering, Tsize, rH, PDI, kD; Laser wavelength: 405 nm ± 5 nm; Concentration range (DLS/SLS): 0.5 mg/mL for a 15 kDa protein, up to 40% w/v; Size resolution: Down to 0.5 nm – 2 μm. |
| SLS | Measurement parameters: Tscattering, average scattering intensity, molecular weight, B22; Measurement accuracy: ≤ 10% molecular weight. |
| Backreflection | Measurement parameters: Tturbidity; Measurement accuracy: Larger than 12.5 nm radius. |