STED超分辨显微成像系统
仪器介绍
STEDYCON是一种新型显微镜设备。它可以安装在传统的落射荧光显微镜，使其成为多功能的四色共焦和2D-STED纳米显微镜。无需搭配共聚焦系统，只需使用现有的宽视场显微镜即可。STEDYCON体积小，但它能获得30nm分辨率的惊人图像，同时还可以通过去卷积进一步提高分辨率。对于新用户来说，操作流程十分友好。
参数
| Objectives | *10X/ 0.4; *20X/ 0.8; *40X oil/ 1.4; *60X oil/ 1.42; *100X oil/ 1.45; Scan field: 80 um* 90 um with 100X objective. |
| Lasers | Ultra-short pulse blue laser: 488 nm, 40 MHz, 500 uW@40 MHz; Ultra-short pulse green laser: 561 nm, 40 MHz, 100 uW@40 MHz; Ultra-short pulse red laser: 640 nm, 40 MHz, 800 uW@40 MHz; STED laser: 775 nm, 1.2W, 40MHz, based on easySTED design. |
| Scan unit | 4 galvanometer scanning unit |
| Scan field | 80 um* 90 um |
| Scan channel | 4 channels in confocal 2 channels in SR |
| Detection bands | 420-480 nm; 505-550 nm; 575-625 nm; 650- 700 nm. |