超分辨显微成像系统
仪器介绍
Elyra 7是一种超分辨率显微镜，能够在大视场、3D、长时程和多通道下观察活样本中的快速细胞过程。它还具有单分子定位（SMLM）功能，用于PALM、dSTORM和PAINT等技术。
参数
| Imaging modes | Widefield Apotome Lattice SIM2 TIRF SMLM (PALM, dSTORM, and PAINT) |
| Objectives | 10X/ 0.3 40X oil/ 1.4 63X oil/ 1.4 63X oil/ 1.46 |
| Lasers | 405 nm (50 mW) 488 nm (500 mW) 561 nm (500 mW) 640 nm (500 mW) |
| Filter sets | LP590 BP490-560+LP630 |
| Cameras | 2x PCO.edge 4.2 sCMOS cameras |
| Incubation system | Temperature adjustable between 28 – 60℃ (Accuracy+/- 0.01℃); CO2 concentration adjustable between 0 – 8% (Accuracy+/- 0.01%); Humidity adjustable between 50-70% under 37℃ |
| Scanner | 1. Widefield mode: 200 nm XY resolution; Up to 5 fps @512 x 512 pixels; 2. Apotome mode: 200 nm XY resolution; Up to 51 sectioned fps @512 x 512 pixels; 5 phase images per one SIM image 3. Lattice SIM mode: 120 nm (XY), 300 nm (Z) resolution; Up to 17 SIM fps @512 x 512 pixels; 15 phase images per one SIM image 4. Lattice SIM2 mode: 60 nm(XY), 200 nm(Z) resolution; 5. SMLM/ TIRF mode: 20 nm - 30 nm (XY), 50 nm – 80 nm (Z) resolution |
| Sample insert | 1x Slide 1x Petri Dish 35 mm, 60 mm; Universal Mounting Frame |
| Software | ZEN 3.5 and ZEN 3.5 desk Multi-dimensional data acquisition Time lapse scan 3D-PALM module Tiles & Positions Experiment Designer |