Single Molecule Localization Super Resolution System
Equipment Introduction
Abbelight SAFe 360 based on single molecule localization technology (SMLM). It can easily superresolution imaging at 15 nm level to observe organelle structure and morphology and the distribution, co-localization of different structural functional proteins. Through its unique ASTER scanning technology to achieve a single acquisition of 180 μm*180μm ultra-large field of vision imaging. Through its single-molecule tracking function, the system can realize the diffusion of small and medium-sized molecules in living cells, reconstruct the transfer trajectory and the diffusion mechanism of small molecules.
Specification
| Multiple single molecule localisation microscopy modality | PALM, STORM, PAINT, Single-Particle Tracking |
| Resolution | xy: 15 nm, z: 20 nm |
| Ultimate illumination mode | Epi, HiLo, TIRF |
| Maximun imaging range | 180 x 180 µm (ASTER technology) |
| Speed | 300 FPS |
| Spectral far-red demixing | Yes |
| Simultaneous multi-color | Yes; Simultaneous 3-color super resolution imaging |
| Cameras | 2x Photometrics Primer BSI sCMOS |
| Lasers | 405 nm, 100 mW； 488 nm, 200 mW； 561 nm, 500 mW； 640 nm, 500 mW； |
| Objectives | 4X/ 0.15； 10X/ 0.4; 20X/ 0.6; 40X/ 0.95; 60X oil/ 1.42; 100X oil/ 1.5 |