Atomic Force Confocal Microscope
Equipment Introduction
The Bruker NanoWizard V atomic force microscope, paired with a ZEISS LSM900 confocal, offers high-resolution imaging and mechanical characterization of biological samples. It features an in-situ cell culture module, force spectroscopy, nanolithography and nanomanipulation functions, as well as a piezoelectric scanner with a 100 μm Z-range for comprehensive analysis of sample morphology, mechanics, and biochemistry, advancing the study of biomechanical properties and cellular functions.
Specification
| NanoWizard® V AFM head | Tip-scanning design Scan range: XY 100 μm×100 μm, Z 15 μm Resolution: Z-noise (in feedback): <30 pm RMS，XY<90 pm RMS sensor noise level Thermal noise cantilever calibration up to 3.25 MHz Equipped with Fast scanner with up to 400 lines/sec speed Supported Modes： 1. Imaging modes in air and liquid: QI Advanced Imaging, PeakForce Tapping™, Quantitative Imaging (QI), PeakForce-QI, Contact mode with lateral force (LFM), TappingMode™ (AC Mode) with Phase Imaging™, Magnetic Force Microscopy (MFM), Electric Force Microscopy (EFM) 2. Spectroscopy modes: Force-distance spectroscopy, other point spectroscopy, Contact Mode Force mapping/Smart Mapping, AC Mode Force Spectroscopy, AC Mode Force mapping, Force Modulation Force Spectroscopy 3. Manipulation modes: Contact Mode Manipulation, AC Mode Manipulation, Force Modulation Manipulation |
| CellHesion® 300 head | 100 µm vertical sensor movement range Contains exchangeable single cell force sensor holder and force measurement system in a rugged, compact chassis Precise force measurements on single cells with single molecule resolution Determination of interaction strength, mechanical response, elasticity, and distances on the nanoscale Fully motorized laser and detector alignment and motorized in-situ switch from air to liquid operation (AutoAlign) |
| Main unit | JPK Vortis 2.1 SPM controller |
| Light source | 980 nm laser |
| Sample stage | Travel range: >20 mm x 20 mm Step size (Resolution): below 1 µm |
| Compatible carriers | Glass slides, Petri dishes, coverslips, metal discs, etc. |
| Align with optical images | JPK DirectOverlay™ 2 software module with camera, communication with ZEISS LSM 900 Confocal Microscope |
| Data acquisition software | JPK SPMControl Software V8 with workflow-based design |
| Data analysis software | JPK DP DataProcessing (DP) software |
| Live-cell system | JPK PetriDishHeater™ (compatible with 35x10 mm Petri dishes from WPI and TPP (plastic or plastic with glass bottom) Ports for perfusion tubes and gas supply |