Cell Sorter
Equipment Introduction
The BD FACSDiscover™ S8 Cell Sorter, featuring BD CellView™ Image Technology and BD SpectralFX™ Technology, is the pioneering spectral flow cytometer sorter with sort-capable image analysis. This revolutionary technology expands the capabilities of cell analysis and sorting by integrating spectral flow cytometry with real-time spatial and morphological insights, enabling scientists to tackle previously unanswerable questions. It allows for real-time visual confirmation of cell populations and characteristics during analysis and sorting, improving spectral flow cytometry with spatial and morphological insights to identify and isolate cell types that were previously inaccessible. Additionally, it simplifies workflows with integrated visual inspection capabilities, flexible panel design, and automated features. Moreover, it enables the creation of comprehensive profiles of highly complex diseases and systems by correlating downstream analysis with imaging and traditional flow data.
Specification
| Lasers | 349 nm (30 mW) 405 nm (50 mW) 488 nm (100 mW) 561 nm (50 mW) 637 nm (100 mW) |
| Scatter detectors | Blue laser: Forward side scatter (FSC) Side scatter (SSC) Axial light loss (ALL) Violet laser: Axial light loss (ALL) Side scatter (SSC) |
| Fluorescence detectors | UV 349-nm laser (22 UV detectors, covering 365–860 nm) Violet 405-nm laser (20 Violet detectors, covering 410–860 nm) Blue 488-nm laser (16 Blue detectors, covering 495–860 nm) Y/G 561-nm laser (12 Yellow-green detectors, covering 570–860 nm) Red 637-nm laser (8 Red detectors, covering 645–860nm) |
| Image-enabled detectors | Blue laser scatter detectors: Forward scatter (FSC) Side scatter (SSC) Axial light loss (ALL) Fluorescence detectors for imaging (PMTs): FL1: LP505: 534/46 FL2: LP570: 600/60 FL3: LP675: 788/225 |
| Imaging features | Center of mass X Center of mass Y Correlation Delta center of mass Diffusivity Eccentricity Max intensity Moment (long) Moment (short) Radial moment Size Total intensity |
| Nozzle size | 85/100/130 µm |
| Sort collection devices | Tube sorting: 1.5-mL microtubes, 2.0-mL tubes and 5.0-mL 12x75-mm tubes Plate sorting: 6-, 24-, 48-, 96- and 384-well plates, microscope slide (optional) |