Fully Automated Immunoassay System
Equipment Introduction
This is a fully automated high-throughput Western blot device. It utilizes capillary electrophoresis combined with patented internal coating technology to automatically complete the entire process from sample loading to electrophoresis separation, immunoblotting, chemical signals acquisition of by a cold CCD camera, and quantification of peak areas through chemiluminescent signal spectra, thus achieving quantitative analysis of Western blot lane images. It supports detection of 1-96 samples, only need 3-5 μL samples and is suitable for analyzing micro-scale samples, such as flow cytometry sorted cells, exosomes, organoids, etc.
Specification
| Sample required | 0.6-1.2 µg |
| Volume required | 3-5 µL/well |
| Size range | Molecular weight (MW) ladders range from 2-440 kDa |
| Sizing CV | <10% |
| Intra-assay CV | <15% |
| Inter-assay CV | <20% |
| Resolution (± percent difference in MW) | ±15-20% for MW <20 kDa, ±10 % for MW > 20 kDa |
| Quantitation CV | <20% |
| Dynamic range | 3 logs |
| Sensitivity | Low pg |
| Capillary | 5 cm, 100 µm, 400 nL |