High-Resolution Inverted Fluorescence Microscope

Model:

Axio Observer 7 with Apotome3

Location:

123C, 1F, E4

Equipment Introduction

Apotome 3 significantly increases the axial resolution compared to conventional fluorescence widefield microscopy: You obtain optical sections that allow 3D rendering, even from thick specimens. Three grids of different geometries give you the best resolution for each objective. You can focus on your experiment as the ideal illumination structure is automatically selected, always resulting in high-contrast optical sections.

Optical sectioning with ZEISS Apotome 3 allows you to efficiently minimize out-of-focus light. Create crisp images and 3D renderings, even of thicker specimen, while the microscope remains just as easy to operate as always. Go one step further with Apotome Plus and achieve confocal-like image quality.

Specification

Light sourceLED multi-color fluorescent light source, 385 nm, 423 nm, 469 nm, 508 nm, 555 nm, 591 nm, and 631 nm
FiltersEx 375/28 nm, Em 460/50 nm;
Ex 470/40 nm, Em 525/50 nm;
Ex 545/25 nm, Em 605/70 nm;
Ex 620/60 nm, Em 700/75 nm;
ObjectivesPlan Fluorescent Phase Contrast Objective Lens, 10x NA: 0.3, WD: 5.2mm;
Plan Apochromatic Fluorescent Objective Lens,  20x NA: 0.8, WD: 0.55mm;
Plan Long Working Distance Fluorescent Phase Contrast Objective Lens, with correction collar, 20x NA: 0.4, WD: 8.4mm;
Plan Apochromatic Fluorescent Oil Immersion Objective Lens, 40x NA: 1.3, WD: 0.21mm;
Plan Apochromatic Fluorescent Oil Immersion Objective Lens, 63x NA: 1.4, WD: 0.19mm;
Camera2048×2048 pixels, 6.5 µm pixel size

Brochure

Axio Observer 7 with Apotome3_Brochure_EN