In-situ Tissue Analysis System
Equipment Introduction
Xenium in situ analysis uses circularizable probes specific to target transcripts followed by enzymatic amplification to create a target for fluorescent probe hybridization. On the Xenium Analyzer, microscope images of the tissue detect the location of each fluorescent probe, which is then removed. Successive rounds of fluorescent probe hybridization, imaging, and removal creates a unique optical signature that reveals the identity of the RNA at a location within each cell of a tissue. In the future, Xenium will allow the detection of both RNA and protein in the same tissue section, revealing complex and nuanced expression patterns.
Specification
| Resolution | -0.2 microns/pixel; <50nm lateral localization precision per image |
| Run time | Up to 3 runs per week |
| Throughput | Up to 2 slides per run:12x24 mm imageable area per slide |
| Sample type | FF and FFPE tissue sections |
| Targets and plexy level | Up to 400 RNA transcripts |