Live Sample Light-sheet Microscope
Equipment Introduction
The LiTone LBS2 employs an ultra-thin linear Bessel light sheet at the objective focal plane to excite samples, achieving low phototoxicity, high-speed, and high-resolution three-dimensional imaging of live cell structures. It offers a lateral resolution of 250 nm and a capture speed of up to 500 frames per second. The observable samples include live adherent cells or tissues, embryos or small animals and suspended cells or microorganisms.
Specification
| Lasers | 405nm (DAPI), 488nm (GFP) 561nm (mCherry) 647nm (Alexa647) |
| Detection objectives | 25x/1.1 Water-immersion lens, WD 2 mm (with 1x & 2.5x optical zoom) |
| Field of view | Single view: 200μm X 200μm (automatic stitching for wide-field range imaging) |
| Sample size | 20 mm X 20 mm X 2 mm |
| Max data acquisition speed | 80 frames per second (2048 X 2048 full frame) |
| Resolution | Lateral resolution: 250nm Axial resolution: 350nm |
| Time resolution | 2 Hz per channel @ sample size of 200 μm X 30 μm X 30 μm |
| Adaptable samples | Fixed or live adherent cells or tissues Embryos or small animals Suspended cells or microorganisms |
| Loading method | horizontal sample loading method |
| Sample stage | High-precision three-axis motorized stage |
| Imaging depth | Typically 30-1500 μm, determined by the optical properties of the samples |
| Environmental control module | Integrated live cell culture chamber, temperature control range: 25-40 ºC, 5% CO2 inlet |