LC-MS
Equipment Introduction
Built on the core principles of its predecessor, the nanoElute 2 has been refined for unrivalled robustness, ultimate chromatographic performance, and ease-of-use. The nanoElute 2 covers a broad range of applications from single-cell to high-throughput routine proteomics. This next generation system provides you with the highest chromatographic resolution resulting in exceptional peptide identification and quantification.
Elute LC systems are built on an innovative pump design that provides a virtually pulse free, precise, volumetric flow control which is independent of flow rates, solvent compressibility or composition.
All Elute LC systems are configured with two pairs of serial-coupled, individually controlled linear drive pump heads. This principle makes it easy to transfer ultra high performance liquid chromatography methods from one system to another, whether to an adjacent laboratory or across the globe.
The flow path is optimized by minimizing the gradient delay volume to deliver high chromatographic resolution, fast gradients and ultimate short cycle times, increasing sample throughput and reducing cost.
The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale. The timsTOF platform stands out for its exceptional reliability and minimal maintenance needs, making it ideally suited for seamless integration into everyday laboratory workflows with maximum uptime and fast turnarounds. More than >7000 protein groups can be identified on average in only 5-minute gradient time with ease and across sites. Achieve top edge reproducibility over time without manual interference.
Specification
| nanoElute | |
| Sample loop | 20 µL |
| Sample loading range | 1-10 µL（Partial loop）or 20 µL (full loop) |
| Sample tray | 2 sample plate (54 position/plate) or 96-well plate or 384-well plate |
| Sampler Temperature range | 15℃ below room temperature |
| Single-acting syringe pump | |
| Flow | 50-2000 nL/min |
| Pressure of loading pump | < 1000 bar |
| Column compartment temperature | 25-60℃ |
| Elute | |
| High-pressure mixing binary pump | max. 1300 bar |
| Flow | 1-5000 µL/min |
| Mixer volume | 35 µL |
| Sample loading mode | Full-loop/partial-loop/µL-pickup |
| Sample tray | 1 sample plate (108 position for 2mL-vial or 30 position for 10mL-vial) or 96-well plate or 384-well plate |
| Sampler temperature range | 4-40℃ |
| Column compartment temperature | 15-90℃ |
| timsTOF HT | |
| Scan rate | MS ≥ 50 Hz; Up to 300 Hz in dda-PASEF |
| MS range | Up to 20000 m/z |
| Resolution | 60000 (1222 m/z) |
| Mass accuracy | Internal ≤ 0.8 ppm (tuning mix m/z 1221.990637, 30 spectral)；External ≤ 2 ppm (tuning mix m/z 1221.990637, 10 spectral) |
| Sensitivity | 1pg/µL Reserpine (m/z 609), S/N 100:1 |
| Features | Trapped Ion Mobility Spectrometry (TIMS) is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF. |