LC-MS
Equipment Introduction
The SCIEX 7500+ system is a state-of-the-art triple quadrupole mass spectrometer engineered to maintain exceptional sensitivity for extended periods in complex matrices. This advanced system elevates precision quantitation, enabling accurate results over prolonged durations and across thousands of sample sets. With the SCIEX 7500+, studies can be conducted more efficiently, providing the confidence to address emerging analytical challenges. Designed for large multiple reaction monitoring (MRM) panels, the SCIEX 7500+ also features enhanced front-end user serviceability, allowing for improved management of instrument downtime. This groundbreaking technology facilitates ultrasensitive quantitation in various applications, including drug development, emerging contaminants, and life sciences research. The performance of the SCIEX 7500+ has undergone rigorous testing with accelerated high-throughput assays on matrices known for their potential to cause instrument contamination. As a result, the system delivers sensitive and robust quantitation, enabling the accurate measurement of ultra-low-level compounds in complex matrices while significantly reducing the risk of downtime.
Specification
| Core ExionLC AE System | |
| Pump | Ultra-High Pressure Binary Gradient Pump |
| Flow rate | 0.010 mL/min - 5.000 mL/min |
| Flow rate precision | < 0.075% RSD |
| Pressure range | ≥ 15,000 psi |
| Column oven | Ambient temperature - 85°C |
| Sample tray | Accommodates over 160 positions, compatible with 1.5 mL or 2 mL sample vials. |
| Injection volume | 0.1 - 50 µL |
| Sampler temperature range | Equipped with cooling function, temperature control from 4°C to 40°C. |
| SCIEX 7500+ | |
| Scan range | Low mass≥5 amu, high mass≤2000 amu |
| Mass accuracy | ≤ 0.1amu/24 hr |
| Dynamic range | ≥ 6 order |
| Resolution | ≥ 9000 |
| Positive and negative polarity switching time | ≤ 5 ms |
| MRM dwell time | ≥ 0.5 ms |
| MRM acquisition speed | 800 MRM/s |
| Sensitivity | •ESI+ sensitivity: 1 pg reserpine directly injected, MRM ion pairs m/z 609 and 195, resolution set to 0.6-0.8 amu (FWHH), signal-to-noise ratio ≥ 3,000,000:1. •ESI- sensitivity: 1 pg chloramphenicol directly injected, MRM ion pairs m/z 321 and 152, resolution set to 0.6-0.8 amu (FWHH), signal-to-noise ratio ≥ 3,000,000:1. |
| Scan mode | Including but not limited to full scan, product ion scan, precursor ion scan, neutral loss scan, multiple reaction monitoring (MRM) scan, automatic MS/MS scan, selective ion monitoring, time programming, positive/negative polarity switching, and MRM3 scanning mode. |