Live Cell Imaging System
Equipment Introduction
Celldiscoverer 7 (CD7) is an automated inverted microscope with an integrated darkroom for the observation of live and fixed samples. Users can perform high-content phase-contrast and fluorescence imaging (done via LED excitation) on CD7.
Specification
| Objecticves | 5X/ 0.35; 20x/ 0.7, autocorrect, long working distance≥2.2 mm; 20x/ 0.95, autocorrect; 50x/ 1.2, water, autocorrect; Temperature of each objective can be adjusted according to the sample temperature; With afocal magnification changer: 0.5x/1x/2x. |
| LEDs for fluorescence excitation | 385/ 420/ 470/ 520/ 567/ 590/ 625 nm |
| Filter sets | 92 HE: RTBS 405 + 493 + 610; EM TBP 425/30 + 524/50 + 688/145 91 HE: RTBS 450 + 538 + 610; EM TBP 467/24 + 555/25 + 687/145 90 HE: RBQS 405 + 493 + 575 + 653; EM QBP 425/30 + 514/30 + 592/25 + 709/100 |
| Cameras | Axiocam 506 mono, Number of Pixels: 2752 (H) x 2208 (V) = 6 Mega, Pixels Pixel size: 4.54 µm x 4.54 µm Orca Flash 4.0 v3, Number of Pixels: 2048 (H) x 2048 (V) = 4 Mega, pixel Pixel size: 6.5 µm x 6.5 µm |
| Incubation system | CO2 Module S1 CO2 concentration adjustable between 1-8%, Precision±0.1% TempModule S1 Temperature adjustable between 28 - 45 °C, Accuracy±0.1% |
| Stage | Scanning stage 300 x140 mm(XY) |
| Sample insert | *1x Petri Dish 35mm 2x Slide - 76x26mm 2x Chamber 2x - Short 2x Chamber 4x - Short Multichamber 1536, 384, 96 *1x Petri Dish 60mm 2x Slide - 57x26mm 2x Chamber 8x - Short 6x Petri Dish 35mm Multiwell 12, 1536, 24, 384, 48, 6, 96 |
| Software | ZEN Blue 3.5 - includes the following modules: Deconvolution 3Dxl Intellesis Segmentation Macro Environment Guided Acquisition Experiment Designer |