Multi-Mode Microplate Reader
Equipment Introduction
BioTek Synergy Neo2 multimode microplate reader features patented Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements. This HTS multimode reader features advanced environmental controls, including CO2/O2 control, incubation to 70 °C, and variable shaking support live cell assays. The compatible BioStack Neo delivers walk-away automation, high throughput, barcode-labeled filter cubes to streamline workflows and limit errors. Powerful Gen6 software provides complete reader control and powerful data analysis.
Specification
| Detection modes | UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Alpha |
| Light sources | Three xenon flash lamps for UV-Vis absorbance, fluorescence intensity, fluorescence polarization, and time-resolved fluorescence; 337 nm laser for time-resolved fluorescence; 680 nm laser for Alpha detection |
| Detectors | Four PMTs Silicon photodiode (absorbance) |
| Read methods | End point, kinetic, spectral scanning, well-area scanning |
| Labware | 6- to 1536-well plates Take3 microvolume plates Petri and cell culture dishes |
| Read path | Top reading and bottom measurements with monochromator- and filter-based optics: fluorescence intensity, fluorescence, polarization, time-resolved fluorescence, and luminescence; Top only: absorbance and Alpha measurements |
| Environmental controls | 4-Zone incubation to 70°C with Condensation Control CO2/O2 controller |
| Reagent injector | Two syringe pump injector module |
| Shaking | Linear, orbital, double orbital |