Realtime PCR System
Equipment Introduction
The LightCycler 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.
Specification
| Temperature control | Peltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses) . |
| Reaction volumes | 5 μL–20 μL (384-well) 10 μL–100 μL (96-well) |
| Heating rate | 96-well block: 4.4°C/s 384-well block: 4.8°C/s |
| Cooling rate | 96-well block: 2.2°C/s 384-well block: 2.5°C/s |
| Excitation | LightCycler® 480 LED Lamp (430-630 nm) |
| Detector | Cooled monochrome CCD camera |
| Filters | Excitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660 |
| Applications | Relative and absolute quantification Qualitative analysis Endpoint SNP genotyping Melting Curve / HRM |
| Reference | No need for passive reference dyes (ROX) or normalization plates. |
| Probe formats | FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes |
| Fluorescent dyes | SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler® Red 610, LightCycler® Red 640, and LightCycler® CYAN 500 |
| Plate formats | 96-and 384-well format (flexibly switch between plate formats and thermo blocks) |
Brochure
LightCycler 480 II_Brochure_EN