Single-cell Western Blotting Platform
Equipment Introduction
The single-cell western blotting platform Milo performs qualitative and quantitative analysis of protein expression at the single-cell level, through fluorescent-labeled antibodies for hybridization and elution, and then detecting the target protein by fluorescence imaging. It is suitable for validating single-cell sequencing results at the protein level, analyzing protein co-expression at the single-cell level, and measure proteins limited by flow cytometry that have no good flow antibodies. It can detect the expression differences of proteins between cells in complex cell populations.
Specification
| Sample type | Suspension containing >10,000 cells |
| Cell diameter | 7-25 µm in suspension |
| Cell type | Mammalian cells; globular in suspension and unfixed |
| Antibody requirement | Standard unlabeled primaries and fluorescent secondaries |
| Typical cell dilutions | Yield capture and analysis of 1,000-2,000 cells per scWest chip |
| Molecular weight (MW) range | 15-175 kDa |
| MW resolution | 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel |
| Typical target multiplexing | Up to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing |
| Workflow time | 4-6 hours |