Small Animal Functional Ultrasound Imaging System
Equipment Introduction
Iconeus One, a functional ultrasound (fUS) imaging system, utilizes innovative multi-plane wave (MPW) ultrasound technology to achieve 10-ms temporal resolution and 100-μm spatial resolution. The Ultrasound Localization Microscopy (ULM) function enables 5μm ultra-high resolution and mm/s-range blood velocity quantification with microbubble. Equipped with 64, 128, and 256-channel low-noise, ultra-light probes and a four-axis motorized moving device for head-fixed animals, it allows automated, multi-angle scanning and imaging. The built-in Allen Mouse Brain Reference Atlas automatically identifies and labels brain regions, streamlining data analysis. Suitable for various animal models, from rodents to non-human primates, Iconeus One performs real-time, large field-of-view, highly sensitive, 2D/3D brain functional imaging in both head-fixed and freely-moving states, making it a versatile tool for neuroscience research.
Specification
| Main unit | ICONEUS-ONE 256 channel |
| Probes | a）IcoPrime-4D MultiArray 15/256: for Head-fixed mice, multi-slice b) IcoPrime 15/128: for both head-fixed and mobile animals, single slice c) IcoPrime-Lite 15/128: for Mobile rats, single slice d) IcoPrime-Mini 15/64: for Mobile mice, single slice |
| Scanning platform | Four-axis motorized scanning platform for anesthetized or head fixed animals |
| Super resolution module | With specific imaging sequence for data acquisition and calculation module for post processing, Ultrasound Localization Microscopy (ULM) uses fUS to track injected microbubbles within blood vessels. This allows images of the micro-vasculature to be generated, with resolution as low as 5 µm. |
| Spatial resolution | Default mode: 100 × 100 × 400 μm at 15 MHz, with depth of ~1.5 cm ULM mode: Imaging micro-vasculature with resolution as low as 5 µm. |
| Sofeware | a) IcoScan：Data live visualization and high-sensitivity single/multi-slice acquisition, 2D/3D angiography, B-mode imaging option. b) IcoStudio: Neuro-navigation function with automatic atlas registration (Allen Mouse Brain Reference Atlas integrated) and streamlined probe positioning; 2D/3D data visualization and analysis for functional ultrasound including activation map, relative CBV changes and functional connectivity. c) IcoLab: Batch processing for functional ultrasound analysis, including seed mapping and connectivity matrices, with report generation; Configurable workflows for ULM map computation for advanced vascular super-resolution studies. |