Super-resolution Microscope
Equipment Introduction
Elyra 7 is a super-resolution microscope that enables the examination of fast cellular processes in live samples – in large fields of view, in 3D, over long time periods, and with multiple colors. It also features Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.
Specification
| Imaging modes | Widefield Apotome Lattice SIM2 TIRF SMLM (PALM, dSTORM, and PAINT) |
| Objectives | 10X/ 0.3 40X oil/ 1.4 63X oil/ 1.4 63X oil/ 1.46 |
| Lasers | 405 nm (50 mW) 488 nm (500 mW) 561 nm (500 mW) 640 nm (500 mW) |
| Filter sets | LP590 BP490-560+LP630 |
| Cameras | 2x PCO.edge 4.2 sCMOS cameras |
| Incubation system | Temperature adjustable between 28 – 60℃ (Accuracy+/- 0.01℃); CO2 concentration adjustable between 0 – 8% (Accuracy+/- 0.01%); Humidity adjustable between 50-70% under 37℃ |
| Scanner | 1. Widefield mode: 200 nm XY resolution; Up to 5 fps @512 x 512 pixels; 2. Apotome mode: 200 nm XY resolution; Up to 51 sectioned fps @512 x 512 pixels; 5 phase images per one SIM image 3. Lattice SIM mode: 120 nm (XY), 300 nm (Z) resolution; Up to 17 SIM fps @512 x 512 pixels; 15 phase images per one SIM image 4. Lattice SIM2 mode: 60 nm(XY), 200 nm(Z) resolution; 5. SMLM/ TIRF mode: 20 nm - 30 nm (XY), 50 nm – 80 nm (Z) resolution |
| Sample insert | 1x Slide 1x Petri Dish 35 mm, 60 mm; Universal Mounting Frame |
| Software | ZEN 3.5 and ZEN 3.5 desk Multi-dimensional data acquisition Time lapse scan 3D-PALM module Tiles & Positions Experiment Designer |