Two-photon Microscopy lmaging System
Equipment Introduction
The OLYMPUS FVMPE-RS two-photon microscopy system is equipped with a 60x and an infrared-corrected 25x high-NA water immersion objective, along with high-power femtosecond lasers ranging from 690 nm-1040 nm and 690 nm-1300 nm, enabling multicolor two-photon imaging. The Inner Focus rotating objective allows seamless switching between upright and inverted microscope configurations, providing flexibility for various live tissue imaging applications. The resonant scanner achieves high-speed imaging up to 438 FPS, capturing fast dynamic processes. The SIM light-stimulation module enables spatially and temporally precise stimulation synchronized with two-photon imaging, significantly enhancing temporal resolution. The FVMPE-RS is a powerful and versatile multiphoton microscopy system, serving as a valuable tool for research in neuroscience, developmental biology, and other fields.
Specification
| Objectives | *5X /0.1, WD 20 mm *60X water/1.1, WD 1.5 mm *25X water/1.05, WD 2 mm, infrared-specific objective with spherical aberration correction ring *25X water/1.0, WD 8 mm, multiphoton-specific high refractive index objective, correction wavelength 400-1600 nm |
| Lasers | Femtosecond pulse laser: 690-1040 nm & 690-1300 nm |
| Scanner | Galvano & Resonance scanner |
| Optical stimulate module | SIM |
| Speed | 30 FPS@512 X 512 438 FPS@512 X 32 |
| Fliters | BP 495-540 BP 410-460 BP 575-645 BP 520-560 BP 660-750 |
| Rotatable objective | Inner Focus freely rotatable objective |
| Air-lifted cage | Neurotar Mobile HomeCage |