High Content Screening System

Equipment Introduction

CellVoyager CV8000 High Content Screening System combining Yokogawa's proprietary high speed confocal scanner technology, water immersion lenses, up to four high field of view cameras, a microscope stage with cell culture environment, and an integrated robotic pipetter, the system provides high throughput, high resolution imaging and phenotypic screening, making it the ideal choice for a wide range of applications.

Specification

Main unit	• Microlens enhanced dual wide Nipkow disk confocal （pinhole size：25μm/50μm） • 405nm/445nm/488nm/561nm/640nm laser • 365nm LED Illumination • Standerd sCMOS X 4 - 4 mega pixel (2000x2000) - 16 bit resolution - 6.5 μm pixel size - supports fast recording mode with up to 100 fps - QE≥82% • Sample vessel for Microplate(6,24,96,384,1536 well) • Laser autofocus • Software autofocus • CV8000 software(Measurement and analysis) • Bright Field/Phase Contrast • Chambered Type Stage Attachment • 6 objective lens position holder
Objective lens	• 4xDry(NA=0.16)UPLXAPO4X • 10xDry(NA=0.40)UPLXAPO10X2 • 20xDry(NA=0.80)UPLXAPO20X • 40xDry(NA=0.95)UPLXAPO40X2 • 20x Water (NA=1.00 UPLSAPO20XW • 60x Water (NA=1.2) UPLSAPO60XW
Dispenser	• For 384-tip Type - Dispenser, - Tip Rack Loader, - Source Shuttle
Living cell system	• Stage heater • Internal Gas mixture（CO2 & N2） • Humidity maintenance system
Filter	• 5-position filter wheel for each sCOMS • EM 445/45 for 405nm • EM 525/50 for 488nm • EM 600/37 for 561nm • EM 676/29 for 640nm • EM 483/32 for CFP • EM 539/30 for YFP
Advanced imaging control module	• Cell Voyager ACE *The Cell Voyager ACE module enable a fully automated workflow of low magnification pre-scan, image analysis and higher magnification rescan.This workflow can be used, for example, to locate 3D organoids in all wells of a multi-plate, then acquire a z-stack at high power objective lens in the center of the tissue. • Fast Time-lapse module (up to 45fps) *This module enables ultra-high speed continuous fluorescence imaging of a specific field of view and can be applied to time lapse photography of rapid biological reactions such as calcium ion flow, calcium spark, ion channel rapid response, etc
Barcode reader & Sample holder	• For Slide Glass，4 slides (ISO 8037/1)
Software & Workstations	• Standerd WS with CV8000 control system • Advanced Professional image analysis workstation • Cell Path finder High content software -3D cell segmentation module -Aplication mode/ Template mode for different analysis needs -Basic / Advanced cell segmentation mode -Opening algorithms：Custom image analysis • Digital phase contrast analysis module • Machine learning module -Cell recognition mode -Object recognition mode -Up to 10 graphic libraries • Cell tracking module • Deep Learning license -Deep Area Finder -Deep Detection -Deep Image Gate -Deep Image Response

Brochure

Cell Voyager CV8000_Brochure_EN