Isothermal Titration Calorimetry
Equipment Introduction
MicroCal PEAQ-ITC is designed for ease-of-use and exceptional sensitivity. The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). With minimal sample preparation and system optimization data is generated quickly and easily. The wide affinity range enables analysis of weak to high affinity binders, with excellent reproducibility.
Specification
| Measurement type | Affinity (KD), Enthalpy ∆H, Entropy ∆S, Stoichiometry (n) |
| Sample volume | 280 µL |
| Cell volume | 200 µL |
| Injection syringe volume | 40 µL |
| Injection volume precision | < 1% @ 2 µL |
| Sample throughput | 0 - 12 per 8 h day |
| Noise | 0.15 ncal/s |
| Temperature range | 2°C to 80°C |
| Feedback modes | Multiple feedback modes: none, high gain, low gain. |